Journal: International Journal of Molecular Sciences

Article Title: Evaluation of Various Alternative Economical and High Throughput SARS-CoV-2 Testing Methods within Resource-Limited Settings

doi: 10.3390/ijms232214350

Figure Lengend Snippet: Comparison of the sensitivity and specificity of the commercial SARS-CoV-2 RT-PCR assay kits.

Article Snippet: Five commercially available RT-PCR SARS-CoV-2 assay kits from different manufacturers were selected in this study for the method comparison including the Thermo Fisher TaqPathTM COVID-19 Assay Kit (Thermo Fischer Scientific, Pleasanton, CA, USA), an internationally approved kit, and the four alternative RT-PCR SARS-CoV-2 assay kits: Nucleic Acid COVID-19 Test Kit (SARS-CoV-2) (Wuhan Easy-diagnosis Biomedicine, Wuhan, China), abTES TM COVID-19 qPCR I Kit (Anatech Instrument (PTY) LTD, Meadowbrook, Business Estate, Sloane Park, Gauteng, South Africa), PCL COVID19 Speedy RT-PCR Kit (PCL Inc. Multiplex In Vitro Diagnostic Global Leader, Seoul, South Korea), and the PCLMD nCoV One-Step RT-PCR Kit (PCL Inc. Multiplex In Vitro Diagnostic Global Leader, Seoul, South Korea).

Techniques: Comparison, One Step RT-PCR

Journal:

Article Title: Pathological Role of Large Intestinal IL-12p40 for the Induction of Th2-Type Allergic Diarrhea

doi:

Figure Lengend Snippet: Primers and Probes Used for Qualitative and Quantitative RT-PCR

Article Snippet: 27,28 The sequences of primers and probes for real-time PCR were designed by Nihon Gene Research Laboratories (Sendai, Japan) .

Techniques: Real-time Polymerase Chain Reaction

Journal: Mediators of Inflammation

Article Title: A Peptide Analogue of Selectin Ligands Attenuated Atherosclerosis by Inhibiting Monocyte Activation

doi: 10.1155/2019/8709583

Figure Lengend Snippet: IELLQAR decreases PLCA-induced atherosclerosis and impaired Ly-6C high monocyte recruitment to the arterial walls of ApoE −/− mice. Eight-week-old male ApoE-deficient mice randomized into four treatment groups, sham group, normal saline (NS), and two IELLQAR treatment groups (1 and 3 mg/kg), and fed a normal chow diet for 4 weeks. In PLCA surgery, three (left external carotid, internal carotid, and occipital artery) of the four branches of ApoE −/− mice were ligated, and only the superior thyroid artery was left to provide blood circulation. (a) Representative histological analysis results of the carotid artery following H&E staining ( n ≥ 5). Scale bar: 50 μ m. (b) Flow cytometric dot plots showing the percentage of CD45 + CD11b + Ly-6C high monocytes in circulation. (c) Representative histological analysis results of the carotid artery following immunofluorescence staining with Ly-6C ( n ≥ 5). Scale bar: 50 μ m and 25 μ m, respectively. (d) The mRNA expression levels of MCP-1, CCR2, and ICAM-1 in carotid artery tissue were measured by qRT-PCR ( n ≥ 5). Data are presented as the mean ± SEM; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Primer sequences for quantitative real-time polymerase chain reaction (qRT-PCR) analysis were designed and purchased from GenePharma Co. Ltd. (Shanghai, China).

Techniques: Staining, Immunofluorescence, Expressing, Quantitative RT-PCR

Journal: Mediators of Inflammation

Article Title: A Peptide Analogue of Selectin Ligands Attenuated Atherosclerosis by Inhibiting Monocyte Activation

doi: 10.1155/2019/8709583

Figure Lengend Snippet: IELLQAR inhibited monocyte activation by P-selectin-dependent activation of the NF- κ B pathways. (a) PBMCs were treated with P-selectin (10 μ g/mL) for different times. Representative Western blots showing the expression levels of total and phosphorylated I κ B α and P65. (b) PBMCs were treated with IELLQAR (1, 3, or 10 μ M) and coincubated with or without 10 μ g/mL of P-selectin for 30 min. Representative Western blots showing the expression levels of total and phosphorylated I κ B α and P65. (c) PBMCs were treated with PBS, LPS (1 μ g/ml), P-selectin, IELLQAR (10 μ M) plus P-selectin, or IELLQAR plus LPS for 2 h, and the mRNA expression levels of IL-6, TNF- α , and MCP-1 were measured by qRT-PCR. Data are presented as the mean ± SEM ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Primer sequences for quantitative real-time polymerase chain reaction (qRT-PCR) analysis were designed and purchased from GenePharma Co. Ltd. (Shanghai, China).

Techniques: Activation Assay, Western Blot, Expressing, Quantitative RT-PCR